This document provides a summary of findings for a research project aimed at enabling the transition of whole mitochondrial genome sequencing from a research environment to that of forensic laboratories responsible for casework.
The author provides a brief summary of research which demonstrates how massively parallel sequencing (MPS) can significantly increase the value of mitochondrial DNA (mtDNA) sequencing for identification of unidentified human remains and analysis of hairs in traditional forensic casework. The research project had seven primary goals: to formally train Technical Leaders and Analysts, and to document the training process; to perform internal validation of the analytical process; to develop a working standard operating procedure (SOP); to develop accompanying tools, worksheets, data output reports for the mitochondrial genome (mtGenome) sequencing process; to institute appropriate Quality Assurance practices; to perform concordance testing with MPS and Sanger Sequencing methods; and, finally, to implement mtGenome sequencing. The author reports success in all activity areas and provides a detailed description of accomplishments, workflows, and research results. The project summary document also includes a listing of sources cited, products produced such as presentations and publications, and a discussion of the research project’s impact in the criminal justice system as well as contributions to crime laboratories and technology transfer.
Downloads
Similar Publications
- Identifying and Embedding Brokers into a Multi-tiered System of Services to Reduce the Bystander Effect Leading to a Reduction in School Violence: End of Grant Report
- A New Measure of Prevalence for the National Crime Victimization Survey
- The Empirics of Immigration and Homicide: Evidence from California and Texas